Cell Biology Lab Manual - 5674 Words

Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique page 6-7 * Media Preparation page 8-9 * Seed cells page 10 * Subculture page 11-12 * Cell count page 13-14 * Cryopreservation page 15 III. Laboratory Experiments * Intentional Contamination page 16-17†¦show more content†¦A. They are immortal B. They are epithelial cells C. They are cancer cells D. All of the above 3) What is an advantage of cell culture? A. Control the physiochemical environment of an experiment B. It is more fun than anti cell culture C. It is easier than anti cell culture D. All of the above Answers: 1) C 2) D 3) A Introduction to Sterile Technique * What is Sterile Technique? Sterile technique is the process of executing cell culture procedures without introducing contaminating microorganisms (bacteria) from the environment * Sterilize vs Sanitize The difference between sanitization and sterilization is that when you sanitize something, you are getting rid of MOST of the live bacteria. It takes about 30 seconds and we perform this using 70% ethanol or Lysol. When we sterilize something, we make free of ALL live bacteria. Sterilization is done with an autoclave or by gas sterilize (this is not done in lab). -Something that is sterilized is also considered sanitized, but if something is sanitized, it is not sterile. * Personal Hygiene By definition, personal hygiene is the act of grooming, cleaning, and looking after our bodies. In cell biology lab, personal hygiene will be necessary in order to keep contaminants from your body from reaching your cells. 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